The Cytotoxicity Assessment of Novel Formulation Developed to Reduce Dentin Hypersensitivity Utilizing Dehydrogenase Assay

The problem of real treatment teeth hypersensitivity is still important and unsolved. main goal the experiment was to calculate possible toxic effects on fibroblasts cells CCL-1™ (NCTC clone 929) caused by original preparation reduce tooth surfaces’ hypersensitivity, compared marketable Seal & Protect (Dentsply). assessment made through measuring lactate dehydrogenase (LDH assay). Lactate releases from cell’s cytoplasm culture medium as a result cell membrane damage lysis cells. measurement based an ability LDH oxidize lactic acid pyruvic acid, which dependent increase release level. activity in supernatants cultures shows relationship with percentage dead (increased cytotoxicity correlates increasing content cells). In assay, both formulations evaluated after 24 h obtained results were located below control values. After seven days, mean values assay are measurable lower for formulation comparison commercial one at dilution 1:5. At 1:10 ratio, they comparable within range accepted maximal 1:15, higher experimental formulation. polymerization process beneficial test case tested preparations. Average preparations attain acceptable level less than 22.6 ± 8.1%, reliant degree remark time. Original characterized greater homogeneity results. has larger diversity effects, time observation attenuation; however, when paralleled conducted days. This should not have disastrous effect pulp, expected ranges. allows assume that will be introduce stage clinical trials future.